Hepatitis Monthly

Published by: Kowsar

Hepatitis C Virus Capsid Protein and Intracellular Lipids Interplay and itsAssociation With Hepatic Steatosis

Muhammad Sohail Afzal 1 , 2 , * , Najam Us Sahar Sadaf Zaidi 2 , Jean Dubuisson 1 and Yves Rouille 1
Authors Information
1 Center for Infection and Immunity of Lille (CIIL), Institut Pasteur de Lille, Univ Lille Nord de France, Lille, France
2 Atta ur Rahman School of Applied Biosciences, National University of Sciences and Technology, H-12 Islamabad, Pakistan
Article information
  • Hepatitis Monthly: August 01, 2014, 14 (8); e17812
  • Published Online: August 11, 2014
  • Article Type: Research Article
  • Received: January 24, 2014
  • Revised: March 9, 2014
  • Accepted: May 8, 2014
  • DOI: 10.5812/hepatmon.17812

To Cite: Afzal M S, Zaidi N U S S, Dubuisson J, Rouille Y. Hepatitis C Virus Capsid Protein and Intracellular Lipids Interplay and itsAssociation With Hepatic Steatosis, Hepat Mon. 2014 ; 14(8):e17812. doi: 10.5812/hepatmon.17812.

Copyright © 2014, Kowsar Corp. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Angulo P. Nonalcoholic fatty liver disease. N Engl J Med. 2002; 346(16): 1221-31[DOI][PubMed]
  • 2. Patel JH, Cobbold JF, Thomas HC, Taylor-Robinson SD. Hepatitis C and hepatic steatosis. QJM. 2010; 103(5): 293-303[DOI][PubMed]
  • 3. Hwang SJ, Luo JC, Chu CW, Lai CR, Lu CL, Tsay SH, et al. Hepatic steatosis in chronic hepatitis C virus infection: prevalence and clinical correlation. J Gastroenterol Hepatol. 2001; 16(2): 190-5[PubMed]
  • 4. Rubbia-Brandt L, Fabris P, Paganin S, Leandro G, Male PJ, Giostra E, et al. Steatosis affects chronic hepatitis C progression in a genotype specific way. Gut. 2004; 53(3): 406-12[PubMed]
  • 5. Adinolfi LE, Gambardella M, Andreana A, Tripodi MF, Utili R, Ruggiero G. Steatosis accelerates the progression of liver damage of chronic hepatitis C patients and correlates with specific HCV genotype and visceral obesity. Hepatology. 2001; 33(6): 1358-64[DOI][PubMed]
  • 6. Mirandola S, Bowman D, Hussain MM, Alberti A. Hepatic steatosis in hepatitis C is a storage disease due to HCV interaction with microsomal triglyceride transfer protein (MTP). Nutr Metab (Lond). 2010; 7: 13[DOI][PubMed]
  • 7. Afzal MS, Zaidi NSS, Dubuisson J, Rouillé Y. Role of HCV Capsid Protein on Cellular Lipid Droplets Content and Localization during HCV Infection. Int J Biosci Biochem Bioinforma. 2014; 4(1): 19-21
  • 8. Boulant S, Vanbelle C, Ebel C, Penin F, Lavergne JP. Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. J Virol. 2005; 79(17): 11353-65[DOI][PubMed]
  • 9. Boulant S, Montserret R, Hope RG, Ratinier M, Targett-Adams P, Lavergne JP, et al. Structural determinants that target the hepatitis C virus core protein to lipid droplets. J Biol Chem. 2006; 281(31): 22236-47[DOI][PubMed]
  • 10. Miyanari Y, Atsuzawa K, Usuda N, Watashi K, Hishiki T, Zayas M, et al. The lipid droplet is an important organelle for hepatitis C virus production. Nat Cell Biol. 2007; 9(9): 1089-97[DOI][PubMed]
  • 11. McLauchlan J, Lemberg MK, Hope G, Martoglio B. Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets. EMBO J. 2002; 21(15): 3980-8[DOI][PubMed]
  • 12. Delgrange D, Pillez A, Castelain S, Cocquerel L, Rouille Y, Dubuisson J, et al. Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins. J Gen Virol. 2007; 88: 2495-503[DOI][PubMed]
  • 13. Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium. Cancer Res. 1982; 42(9): 3858-63[PubMed]
  • 14. Rouille Y, Helle F, Delgrange D, Roingeard P, Voisset C, Blanchard E, et al. Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus. J Virol. 2006; 80(6): 2832-41[DOI][PubMed]
  • 15. Clement S, Pascarella S, Negro F. Hepatitis C virus infection: molecular pathways to steatosis, insulin resistance and oxidative stress. Viruses. 2009; 1(2): 126-43[DOI][PubMed]
  • 16. Boulant S, Douglas MW, Moody L, Budkowska A, Targett-Adams P, McLauchlan J. Hepatitis C virus core protein induces lipid droplet redistribution in a microtubule- and dynein-dependent manner. Traffic. 2008; 9(8): 1268-82[DOI][PubMed]
  • 17. Binder M, Sulaimanov N, Clausznitzer D, Schulze M, Huber CM, Lenz SM, et al. Replication vesicles are load- and choke-points in the hepatitis C virus lifecycle. PLoS Pathog. 2013; 9(8)[DOI][PubMed]
  • 18. Matto M, Rice CM, Aroeti B, Glenn JS. Hepatitis C virus core protein associates with detergent-resistant membranes distinct from classical plasma membrane rafts. J Virol. 2004; 78(21): 12047-53[DOI][PubMed]
  • 19. Okamoto K, Mori Y, Komoda Y, Okamoto T, Okochi M, Takeda M, et al. Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation. J Virol. 2008; 82(17): 8349-61[DOI][PubMed]
  • 20. Alsaleh K, Delavalle PY, Pillez A, Duverlie G, Descamps V, Rouille Y, et al. Identification of basic amino acids at the N-terminal end of the core protein that are crucial for hepatitis C virus infectivity. J Virol. 2010; 84(24): 12515-28[DOI][PubMed]
  • 21. Popescu CI, Callens N, Trinel D, Roingeard P, Moradpour D, Descamps V, et al. NS2 protein of hepatitis C virus interacts with structural and non-structural proteins towards virus assembly. PLoS Pathog. 2011; 7(2)[DOI][PubMed]
  • 22. Afzal MS, Elreheem AA, Ragaa A, Wahid A. HCV Genotype 4; A Brief Review. J Gastroenterol Hepatol Res. 2014; 3(2): 968-76
  • 23. Anjum S, Ali S, Ahmad T, Afzal MS, Waheed Y, Shafi T, et al. Sequence and structural analysis of 3’ untranslated region of hepatitis C virus, genotype 3a, from pakistani isolates. Hepat Mon 2013; 13(5)
  • 24. Anjum S, Wahid A, Afzal MS, Albecka A, Alsaleh K, Ahmad T, et al. Additional glycosylation within a specific hypervariable region of subtype 3a of hepatitis C virus protects against virus neutralization. J Infect Dis 2013; 208(11): 1888-97
  • 25. Afzal MS, Tahir S, Salman A, Baig TA, Shafi T, Zaidi NU, et al. Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan. J Infect Dev Ctries. 2011; 5(6): 473-9[PubMed]
  • 26. Afzal MS, Anjum S, Zaidi NU. Effect of Functional Interleukin-10 Polymorphism on Pegylated Interferon-alpha Plus Ribavirin Therapy Response in Chronic Hepatitis C Virus Patients Infected With 3a Genotype in Pakistani Population. Hepat Mon. 2013; 13(6)[DOI][PubMed]
  • 27. Flynn JK, Sacks-Davis R, Higgs P, Aitken C, Moneer S, Suppiah V, et al. Detection of HCV-Specific IFN-gamma Responses in HCV Antibody and HCV RNA Negative Injecting Drug Users. Hepat Mon. 2014; 14(1)[DOI][PubMed]
  • 28. Afzal MS, Ahmed T, Zaidi NU. Comparison of HCV prevalence in pakistan and iran; an insight into future. Hepat Mon 2014; 14(1)
  • 29. Afzal MS, Anjum S, Zaidi NU. Changing of HCV clade pattern in iran; the possible means for something good. Hepat Mon. 2014; 14(1)[DOI][PubMed]
  • 30. Kumar D, Farrell GC, Fung C, George J. Hepatitis C virus genotype 3 is cytopathic to hepatocytes: Reversal of hepatic steatosis after sustained therapeutic response. Hepatology. 2002; 36(5): 1266-72[DOI][PubMed]
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