Hepatitis Monthly

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Detection of Hepatitis B Virus Covalently Closed Circular DNA in the Plasma of Iranian HBeAg-Negative Patients With Chronic Hepatitis B

Zahra Tajik 1 , Hossein Keyvani 2 , Farah Bokharaei-Salim 2 , 3 , * , Mohammad Reza Zolfaghari 1 , Shahin Fakhim 4 , Maryam Keshvari 5 and Seyed Moayed Alavian 6 , 7
Authors Information
1 Department of Microbiology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, IR Iran
2 Department of Virology, Iran University of Medical Sciences, Tehran, IR Iran
3 HIV Laboratory of National Center, Deputy of Health, Iran University of Medical Sciences, Tehran, IR Iran
4 Department of Civil Engineering, Faculty of Engineering, Payame Noor University, Karaj, IR Iran
5 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran
6 Middle East Liver Disease Center, Tehran, IR Iran
7 Iran Hepatitis Network, Tehran, IR Iran
Article information
  • Hepatitis Monthly: September 01, 2015, 15 (9); e30790
  • Published Online: September 29, 2015
  • Article Type: Research Article
  • Received: June 15, 2015
  • Revised: August 16, 2015
  • Accepted: August 30, 2015
  • DOI: 10.5812/hepatmon.30790

To Cite: Tajik Z, Keyvani H, Bokharaei-Salim F, Zolfaghari M R, Fakhim S, et al. Detection of Hepatitis B Virus Covalently Closed Circular DNA in the Plasma of Iranian HBeAg-Negative Patients With Chronic Hepatitis B, Hepat Mon. 2015 ; 15(9):e30790. doi: 10.5812/hepatmon.30790.

Copyright © 2015, Kowsar Corp. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Kim BK, Revill PA, Ahn SH. HBV genotypes: relevance to natural history, pathogenesis and treatment of chronic hepatitis B. Antivir Ther. 2011; 16(8): 1169-86[DOI][PubMed]
  • 2. Bokharaei-Salim F, Keyvani H, Monavari SH, Esghaei M, Fakhim S, Ataei Pirkooh A, et al. Distribution of hepatitis B virus genotypes in azerbaijani patients with chronic hepatitis B infection. Hepat Mon. 2014; 14(12)[DOI][PubMed]
  • 3. Schaefer S. Hepatitis B virus genotypes in Europe. Hepatol Res. 2007; 37-6[DOI][PubMed]
  • 4. Olinger CM, Lazouskaya NV, Eremin VF, Muller CP. Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences. Clin Microbiol Infect. 2008; 14(6): 575-81[DOI][PubMed]
  • 5. Tatematsu K, Tanaka Y, Kurbanov F, Sugauchi F, Mano S, Maeshiro T, et al. A genetic variant of hepatitis B virus divergent from known human and ape genotypes isolated from a Japanese patient and provisionally assigned to new genotype J. J Virol. 2009; 83(20): 10538-47[DOI][PubMed]
  • 6. Vaezjalali M, Alavian S, Jazayeri S, Nategh R, Mahmoodi M, Hajibeigi B, et al. Genotype of hepatitis B virus isolates from Iranian chronic carriers of the virus. Hepat mon. 2008; 8(2): 97-100
  • 7. He ML, Wu J, Chen Y, Lin MC, Lau GK, Kung HF. A new and sensitive method for the quantification of HBV cccDNA by real-time PCR. Biochem Biophys Res Commun. 2002; 295(5): 1102-7[PubMed]
  • 8. Wong DK, Yuen MF, Yuan H, Sum SS, Hui CK, Hall J, et al. Quantitation of covalently closed circular hepatitis B virus DNA in chronic hepatitis B patients. Hepatology. 2004; 40(3): 727-37[DOI][PubMed]
  • 9. Guo Y, Sheng S, Nie B, Tu Z. Development of magnetic capture hybridization and quantitative polymerase chain reaction for hepatitis B virus covalently closed circular DNA. Hepat Mon. 2015; 15(1)[DOI][PubMed]
  • 10. Singh M, Dicaire A, Wakil AE, Luscombe C, Sacks SL. Quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in the liver of HBV-infected patients by LightCycler real-time PCR. J Virol Methods. 2004; 118(2): 159-67[DOI][PubMed]
  • 11. Bourne EJ, Dienstag JL, Lopez VA, Sander TJ, Longlet JM, Hall JG, et al. Quantitative analysis of HBV cccDNA from clinical specimens: correlation with clinical and virological response during antiviral therapy. J Viral Hepat. 2007; 14(1): 55-63[DOI][PubMed]
  • 12. Singla B, Chakraborti A, Sharma BK, Kapil S, Chawla YK, Arora SK, et al. Levels of hepatitis B virus replicative intermediate in serum samples of chronic hepatitis B patients. Mol Biol Rep. 2014; 41(7): 4689-96[DOI][PubMed]
  • 13. Ben Slama N, Ahmed SN, Zoulim F. [HBsAg quantification: virological significance]. Gastroenterol Clin Biol. 2010; 34 Suppl 2-8[DOI][PubMed]
  • 14. Chevaliez S, Rodriguez C, Pawlotsky JM. New virologic tools for management of chronic hepatitis B and C. Gastroenterology. 2012; 142(6): 1303-13130[DOI][PubMed]
  • 15. Chen Y, Sze J, He ML. HBV cccDNA in patients' sera as an indicator for HBV reactivation and an early signal of liver damage. World J Gastroenterol. 2004; 10(1): 82-5[PubMed]
  • 16. Lin LY, Wong VW, Zhou HJ, Chan HY, Gui HL, Guo SM, et al. Relationship between serum hepatitis B virus DNA and surface antigen with covalently closed circular DNA in HBeAg-negative patients. J Med Virol. 2010; 82(9): 1494-500[DOI][PubMed]
  • 17. Zhong Y, Han J, Zou Z, Liu S, Tang B, Ren X, et al. Quantitation of HBV covalently closed circular DNA in micro formalin fixed paraffin-embedded liver tissue using rolling circle amplification in combination with real-time PCR. Clin Chim Acta. 2011; 412(21-22): 1905-11[DOI][PubMed]
  • 18. Guner R, Karahocagil M, Buyukberber M, Kandemir O, Ural O, Usluer G, et al. Correlation between intrahepatic hepatitis B virus cccDNA levels and other activity markers in patients with HBeAg-negative chronic hepatitis B infection. Eur J Gastroenterol Hepatol. 2011; 23(12): 1185-91[DOI][PubMed]
  • 19. Zoulim F. New insight on hepatitis B virus persistence from the study of intrahepatic viral cccDNA. J Hepatol. 2005; 42(3): 302-8[DOI][PubMed]
  • 20. Laras A, Koskinas J, Dimou E, Kostamena A, Hadziyannis SJ. Intrahepatic levels and replicative activity of covalently closed circular hepatitis B virus DNA in chronically infected patients. Hepatology. 2006; 44(3): 694-702[DOI][PubMed]
  • 21. Werle-Lapostolle B, Bowden S, Locarnini S, Wursthorn K, Petersen J, Lau G, et al. Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy. Gastroenterology. 2004; 126(7): 1750-8[PubMed]
  • 22. Dehghani SM, Taghavi SA, Geramizadeh B, Nikeghbalian S, Derakhshan N, Malekpour A, et al. Hepatitis B recurrence after liver transplantation: a single center experiences and review the literature. Hepat Mon. 2013; 13(1)[DOI][PubMed]
  • 23. Shao J, Wei L, Wang H, Sun Y, Zhang LF, Li J, et al. Relationship between hepatitis B virus DNA levels and liver histology in patients with chronic hepatitis B. World J Gastroenterol. 2007; 13(14): 2104-7[PubMed]
  • 24. Ganji A, Esmaeilzadeh A, Ghafarzadegan K, Helalat H, Rafatpanah H, Mokhtarifar A. Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV. Hepat Mon. 2011; 11(5): 342-5[PubMed]
  • 25. Serum HBsAg levels reflect intracellular HBsAg, but not cccDNA or total liver HBV DNA in patients with HBeAg-negative chronic hepatitis B. Hepatology. : 969A-70A
  • 26. Lu FM, Zhuang H. Management of hepatitis B in China. Chin Med J (Engl). 2009; 122(1): 3-4[PubMed]
  • 27. Volz T, Lutgehetmann M, Wachtler P, Jacob A, Quaas A, Murray JM, et al. Impaired intrahepatic hepatitis B virus productivity contributes to low viremia in most HBeAg-negative patients. Gastroenterology. 2007; 133(3): 843-52[DOI][PubMed]
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