Hepatitis Monthly

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Design, Construction and Evaluation of 1a/JFH1 HCV Chimera by Replacing the Intergenotypic Variable Region

Faezeh Ghasemi 1 , Majid Ghayour-Mobarhan 2 , Alireza Pasdar 3 , 4 , Hamid Pourianfar 5 , Mohammad Reza Aghasadeghi 6 , Hamed Gouklani 7 , ** and Zahra Meshkat 8 , **
Authors Information
1 Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
2 Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
3 Faculty of Medicine, Department of New Sciences and Technology, Mashhad University of Medical Sciences, Mashhad, IR Iran
4 Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, UK
5 Industrial Biotechnology Research Institute, Iranian Academic Centre for Education, Culture and Research Mashhad Branch, Mashhad, IR Iran
6 Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, IR Iran
7 Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran
8 Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
Corresponding Authors:
Article information
  • Hepatitis Monthly: October 01, 2016, 16 (10); e38261
  • Published Online: August 7, 2016
  • Article Type: Research Article
  • Received: April 6, 2016
  • Revised: July 20, 2016
  • Accepted: July 20, 2016
  • DOI: 10.5812/hepatmon.38261

To Cite: Ghasemi F, Ghayour-Mobarhan M, Pasdar A, Pourianfar H, Reza Aghasadeghi M, et al. Design, Construction and Evaluation of 1a/JFH1 HCV Chimera by Replacing the Intergenotypic Variable Region, Hepat Mon. 2016 ; 16(10):e38261. doi: 10.5812/hepatmon.38261.

Copyright © 2016, Kowsar Corp. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Chambers TJ, Hahn CS, Galler R, Rice CM. Flavivirus genome organization, expression, and replication. Annu Rev Microbiol. 1990; 44: 649-88[DOI][PubMed]
  • 2. Shakeri MT, Nomani H, Ghayour Mobarhan M, Sima HR, Gerayli S, Shahbazi S, et al. The prevalence of hepatitis C virus in mashhad, iran: a population-based study. Hepat Mon. 2013; 13(3): 7723[DOI][PubMed]
  • 3. Poynard T, Yuen MF, Ratzin V, Lai CL. Viral hepatitis C. The Lancet. 2003; 362(9401): 2095-100[DOI]
  • 4. Argentini C, Genovese D, Dettori S, Rapicetta M. HCV genetic variability: from quasispecies evolution to genotype classification. Future Microbiol. 2009; 4(3): 359-73[DOI][PubMed]
  • 5. Yanagi M, St. Claire M, Emerson SU, Purcell RH, Bukh J. In vivo analysis of the 3' untranslated region of the hepatitis C virus after in vitro mutagenesis of an infectious cDNA clone. Proc Natl Acad Sci. 1999; 96(5): 2291-5[DOI]
  • 6. Moradpour D, Penin F, Rice CM. Replication of hepatitis C virus. Nat Rev Microbiol. 2007; 5(6): 453-63[DOI][PubMed]
  • 7. Farci P, Shimoda A, Wong D, Cabezon T, De Gioannis D, Strazzera A, et al. Prevention of hepatitis C virus infection in chimpanzees by hyperimmune serum against the hypervariable region 1 of the envelope 2 protein. Proc Natl Acad Sci U S A. 1996; 93(26): 15394-9[PubMed]
  • 8. Perotti M, Mancini N, Diotti RA, Tarr AW, Ball JK, Owsianka A, et al. Identification of a broadly cross-reacting and neutralizing human monoclonal antibody directed against the hepatitis C virus E2 protein. J Virol. 2008; 82(2): 1047-52[DOI][PubMed]
  • 9. Reed KE, Rice CM. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr Top Microbiol Immunol. 2000; 242: 55-84[PubMed]
  • 10. Albecka A, Montserret R, Krey T, Tarr AW, Diesis E, Ball JK, et al. Identification of new functional regions in hepatitis C virus envelope glycoprotein E2. J Virol. 2011; 85(4): 1777-92[DOI][PubMed]
  • 11. Meshkat Z, Audsley M, Beyer C, Gowans EJ, Haqshenas G. Reverse genetic analysis of a putative, influenza virus M2 HXXXW-like motif in the p7 protein of hepatitis C virus. J Viral Hepat. 2009; 16(3): 187-94[DOI][PubMed]
  • 12. Teimourpour R, Meshkat Z, Gholoubi A, Nomani H, Rostami S. Viral Load Analysis of Hepatitis C Virus in Huh7.5 Cell Culture System. Jundishapur J Microbiol. 2015; 8(5): 19279[DOI][PubMed]
  • 13. Gouklani H, Bull RA, Beyer C, Coulibaly F, Gowans EJ, Drummer HE, et al. Hepatitis C virus nonstructural protein 5B is involved in virus morphogenesis. J Virol. 2012; 86(9): 5080-8[DOI][PubMed]
  • 14. McCaffrey K, Gouklani H, Boo I, Poumbourios P, Drummer HE. The variable regions of hepatitis C virus glycoprotein E2 have an essential structural role in glycoprotein assembly and virion infectivity. J Gen Virol. 2011; 92: 112-21[DOI][PubMed]
  • 15. Gottwein JM, Scheel TK, Hoegh AM, Lademann JB, Eugen-Olsen J, Lisby G, et al. Robust hepatitis C genotype 3a cell culture releasing adapted intergenotypic 3a/2a (S52/JFH1) viruses. Gastroenterology. 2007; 133(5): 1614-26[DOI][PubMed]
  • 16. Lu J, Tao W, Li R, Xiang Y, Zhang N, Xiang X, et al. Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates. Virology. 2013; 443(1): 80-8[DOI][PubMed]
  • 17. Yi M, Ma Y, Yates J, Lemon SM. Compensatory mutations in E1, p7, NS2, and NS3 enhance yields of cell culture-infectious intergenotypic chimeric hepatitis C virus. J Virol. 2007; 81(2): 629-38[DOI][PubMed]
  • 18. Pietschmann T, Kaul A, Koutsoudakis G, Shavinskaya A, Kallis S, Steinmann E, et al. Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras. Proc Natl Acad Sci U S A. 2006; 103(19): 7408-13[DOI][PubMed]
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