Hepatitis Monthly

Published by: Kowsar

Hepatitis B Virus DNA is Absent in Lebanese Blood Donors Who Are Positive for Both Anti-Hbc and Anti-Hbs Antibodies

Ghada Kchour 1 , Mohammad Fayyad-Kazan 2 , * , Antoine Haddad 3 , Bassam Badran 1 and Mahdi Tarhini 4 , *
Authors Information
1 Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, Hadath, Lebanon
2 Institut de Biologie et de Medecine Moleculaires, Universite Libre de Bruxelles, 6041 Gosselies, Belgium
3 Department of Clinical Pathology and Blood Banking, Sacre coeur Hospital, Lebanese University, Lebanon
4 Faculty of Health Sciences V, Lebanese University, and director of Clinical Pathology and Blood Banking Department , Raee Hospital, Saida , Lebanon
Corresponding Authors:
Article information
  • Hepatitis Monthly: February 2017, 17 (2); e44787
  • Published Online: February 7, 2017
  • Article Type: Research Article
  • Received: December 14, 2016
  • Revised: January 11, 2017
  • Accepted: February 4, 2017
  • DOI: 10.5812/hepatmon.44787

To Cite: Kchour G, Fayyad-Kazan M, Haddad A, Badran B, Tarhini M. et al. Hepatitis B Virus DNA is Absent in Lebanese Blood Donors Who Are Positive for Both Anti-Hbc and Anti-Hbs Antibodies, Hepat Mon. 2017 ;17(2):e44787. doi: 10.5812/hepatmon.44787.

Copyright: Copyright © 2017, Hepatitis Monthly. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited..
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Lavanchy D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat. 2004; 11(2): 97-107[DOI][PubMed]
  • 2. European Association For The Study Of The L. EASL clinical practice guidelines: Management of chronic hepatitis B virus infection. J Hepatol. 2012; 57(1): 167-85[DOI][PubMed]
  • 3. Milich DR, Sallberg M, Maruyama T. The humoral immune response in acute and chronic hepatitis B virus infection. Springer Semin Immunopathol. 1995; 17(2-3): 149-66[DOI][PubMed]
  • 4. Jung MC, Pape GR. Immunology of hepatitis B infection. Lancet Infect Dis. 2002; 2(1): 43-50[DOI][PubMed]
  • 5. Laperche S, Guitton C, Smilovici W, Courouce AM. Blood donors infected with the hepatitis B virus but persistently lacking antibodies to the hepatitis B core antigen. Vox Sang. 2001; 80(2): 90-4[DOI][PubMed]
  • 6. Chan HL. Changing scene in hepatitis B serology interpretation. Hosp Med. 2002; 63(1): 16-9[DOI][PubMed]
  • 7. Said ZN. An overview of occult hepatitis B virus infection. World J Gastroenterol. 2011; 17(15): 1927-38[DOI][PubMed]
  • 8. Ramezani A, Banifazl M, Eslamifar A, Aghakhani A. Serological pattern of anti-HBc alone infers occult hepatitis B virus infection in high-risk individuals in Iran. J Infect Dev Ctries. 2010; 4(10): 658-61[PubMed]
  • 9. Kang SY, Kim MH, Lee WI. The prevalence of "anti-HBc alone" and HBV DNA detection among anti-HBc alone in Korea. J Med Virol. 2010; 82(9): 1508-14[DOI][PubMed]
  • 10. Nanu A, Sharma SP, Chatterjee K, Jyoti P. Markers for transfusion-transmissible infections in north Indian voluntary and replacement blood donors: prevalence and trends 1989-1996. Vox Sang. 1997; 73(2): 70-3[DOI][PubMed]
  • 11. Yotsuyanagi H, Yasuda K, Moriya K, Shintani Y, Fujie H, Tsutsumi T, et al. Frequent presence of HBV in the sera of HBsAg-negative, anti-HBc-positive blood donors. Transfusion. 2001; 41(9): 1093-9[DOI][PubMed]
  • 12. Huang CF, Lin SS, Ho YC, Chen FL, Yang CC. The immune response induced by hepatitis B virus principal antigens. Cell Mol Immunol. 2006; 3(2): 97-106[PubMed]
  • 13. Galli C, Orlandini E, Penzo L, Badiale R, Caltran G, Valverde S, et al. What is the role of serology for the study of chronic hepatitis B virus infection in the age of molecular biology? J Med Virol. 2008; 80(6): 974-9[DOI][PubMed]
  • 14. Kleinman SH, Kuhns MC, Todd DS, Glynn SA, McNamara A, DiMarco A, et al. Frequency of HBV DNA detection in US blood donors testing positive for the presence of anti-HBc: implications for transfusion transmission and donor screening. Transfusion. 2003; 43(6): 696-704[DOI][PubMed]
  • 15. Kuhns MC, Busch MP. New strategies for blood donor screening for hepatitis B virus: nucleic acid testing versus immunoassay methods. Mol Diagn Ther. 2006; 10(2): 77-91[DOI][PubMed]
  • 16. Weber B, Melchior W, Gehrke R, Doerr HW, Berger A, Rabenau H. Hepatitis B virus markers in anti-HBc only positive individuals. J Med Virol. 2001; 64(3): 312-9[DOI][PubMed]
  • 17. Manzini P, Girotto M, Borsotti R, Giachino O, Guaschino R, Lanteri M, et al. Italian blood donors with anti-HBc and occult hepatitis B virus infection. Haematologica. 2007; 92(12): 1664-70[DOI][PubMed]
  • 18. Jutavijittum P, Andernach IE, Yousukh A, Samountry B, Samountry K, Thammavong T, et al. Occult hepatitis B infections among blood donors in Lao PDR. Vox Sang. 2014; 106(1): 31-7[DOI][PubMed]
  • 19. Pisano MB, Blanco S, Carrizo H, Re VE, Gallego S. Hepatitis B virus infection in blood donors in Argentina: prevalence of infection, genotype distribution and frequency of occult HBV infection. Arch Virol. 2016; 161(10): 2813-7[DOI][PubMed]
  • 20. Karimi G, Zadsar M, Vafaei N, Sharifi Z, FalahTafti M. Prevalence of antibody to Hepatitis B core antigen and Hepatitis B virus DNA in HBsAg negative healthy blood donors. Virol J. 2016; 13: 36[DOI][PubMed]
  • 21. Ramia S, Ramlawi F, Kanaan M, Klayme S, Naman R. Frequency and significance of antibodies against hepatitis B core (anti-HBc) antigen as the only serological marker for hepatitis B infection in Lebanese blood donors. Epidemiol Infect. 2005; 133(4): 695-9[DOI][PubMed]
  • 22. Katz L, Strong DM, Tegtmeier G, Stramer S. Performance of an algorithm for the reentry of volunteer blood donors deferred due to false-positive test results for antibody to hepatitis B core antigen. Transfusion. 2008; 48(11): 2315-22[DOI][PubMed]
  • 23. Altunay H, Kosan E, Birinci I, Aksoy A, Kirali K, Saribas S, et al. Are isolated anti-HBc blood donors in high risk group? The detection of HBV DNA in isolated anti-HBc cases with nucleic acid amplification test (NAT) based on transcription-mediated amplification (TMA) and HBV discrimination. Transfus Apher Sci. 2010; 43(3): 265-8[DOI][PubMed]
  • 24. Dreier J, Kroger M, Diekmann J, Gotting C, Kleesiek K. Low-level viraemia of hepatitis B virus in an anti-HBc- and anti-HBs-positive blood donor. Transfus Med. 2004; 14(2): 97-103[DOI][PubMed]
  • 25. Lelie N, Bruhn R, Busch M, Vermeulen M, Tsoi WC, Kleinman S, et al. Detection of different categories of hepatitis B virus (HBV) infection in a multi-regional study comparing the clinical sensitivity of hepatitis B surface antigen and HBV-DNA testing. Transfusion. 2017; 57(1): 24-35[DOI][PubMed]
  • 26. Kiely P, Margaritis AR, Seed CR, Yang H, Australian Red Cross Blood Service NG. Hepatitis B virus nucleic acid amplification testing of Australian blood donors highlights the complexity of confirming occult hepatitis B virus infection. Transfusion. 2014; 54(8): 2084-91[DOI][PubMed]
  • 27. Enjalbert F, Krysztof DE, Candotti D, Allain JP, Stramer SL. Comparison of seven hepatitis B virus (HBV) nucleic acid testing assays in selected samples with discrepant HBV marker results from United States blood donors. Transfusion. 2014; 54(10): 2485-95[DOI][PubMed]
  • 28. Lin H, Zhao H, Tang X, Hu W, Jiang N, Zhu S, et al. Serological patterns and molecular characterization of occult hepatitis b virus infection among blood donors. Hepat Mon. 2016; 16(10): 40492
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader