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Associations among Genotype 1b Hepatitis C Virus Core Protein, Protein Kinase R, and Signal Transducer and Activator of Transcription 3

AUTHORS

Xue Bing Yan 1 , * , Zhi Chen 2 , Christian Brechot 3

AUTHORS INFORMATION

1 Department of Infectious Diseases, The First Affiliated Hospital of Xuzhou Medical College, yxbxuzhou@126.com, China

2 Institute of Infectious Diseases, the First Affiliated Hospital, College of Medical Science, Key Laboratory of Ministry Health, Zhejiang University, China

3 INSERM, U785, University Paris sud, Centre Hepato-Biliaire, France

How to Cite: Yan X, Chen Z, Brechot C. Associations among Genotype 1b Hepatitis C Virus Core Protein, Protein Kinase R, and Signal Transducer and Activator of Transcription 3, Hepat Mon. Online ahead of Print ; 10(4):275-284.

ARTICLE INFORMATION

Hepatitis Monthly: 10 (4); 275-284
Article Type: Research Article
Received: February 10, 2010
Accepted: September 17, 2010

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Abstract

Background and Aims: Because hepatitis C virus (HCV) core protein (Core), protein kinase R (PKR), and signal transducer and activator of transcription 3 (STAT3) all play relevant roles in the pathogenesis of HCV, persistent infection and hepatocellular carcinoma (HCC) and PKR may interact with HCV Core. In this study, we further investigate the associations among HCV Core, PKR, and STAT3 and the mechanisms involved in these interactions.
Materials and Methods: Expression levels of HCV Core, PKR, eukaryotic initiation factor 2 (eIF-2α), phosphorylated eIF-2α (p-eIF-2α), STAT3, and phosphorylated-STAT3 (p-STAT3) were compared between Huh-7 and replicon cell-Huh-7 cells harboring the full length of genotype 1b HCV genomes. Co-immunoprecipitation and glutathione S-transferase (GST) pull-down assay were conducted for HCV Core, PKR, and STAT3.
Results: HCV may have induced the expression of STAT3 and the activity of PKR (p-eIF-2α). HCV Core, STAT3, and PKR appear to have interacted with one another. The N-terminal 1-126 amino acid (aa) of HCV Core contributed to an interaction between HCV Core and STAT3, and only full-length PKR bound to STAT3 and p-STAT3.
Conclusions: These findings suggest that HCV Core, PKR, and STAT3 can interact with each other. Specifically, HCV Core may play its role through both PKR and STAT3. Alternatively, HCV Core's binding to and activation of STAT3 might be due to the interaction between HCV Core and PKR. The distinct interactions among these three molecules are important and may reveal a new molecular mechanism in the pathogenesis of HCV-persistent infection and HCV-related HCC.

Keywords

Hepatitis C virus Core protein Protein kinase R Signal transducer transcription 3

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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