Polymorphisms within Fas gene are not associated with occult hepatitis B virus infection

AUTHORS

Mohammad Kazemi Arababadi 1 , * , Adel Mohammadzadeh 2 , Ali Akbar Pourfathollah 2 , Derek Kennedy 3

1 Department of Immunology, School of Medical Sciences, dr.kazemi@rums.ac.ir, Kerman, IR Iran

2 Department of Immunology, School of Medical Sciences, Tarbiat Modarres University, Tehran, IR Iran

3 School of Biomolecular and physical Science, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Australia

How to Cite: Arababadi M, Mohammadzadeh A, Pourfathollah A, Kennedy D. Polymorphisms within Fas gene are not associated with occult hepatitis B virus infection, Hepat Mon. Online ahead of Print ; 11(1):23-26.

ARTICLE INFORMATION

Hepatitis Monthly: 11 (1); 23-26
Article Type: Research Article
Received: March 22, 2010
Accepted: August 11, 2010

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Abstract

Background: Occult hepatitis B infection (OBI) is a form of hepatitis in which there is an absence of detectable HBsAg, despite the presence of HBV-DNA in the peripheral blood of patients. It seems that non-effective or attenuated immune system responses against HBV lead to the development of OBI. Previous studies showed that the Fas/Fas ligand (FasL) system is an important death signaling pathway that is used by cytotoxic T lymphocytes to eradicate HBV from the liver.
Objectives: To investigate polymorphisms in the -670 region of the Fas gene in those with OBI.
Patients and Methods: The plasma samples from 3700 blood donors were tested for HBsAg and anti-HBs by ELISA. The HBsAg-/anti-HBc+ samples were selected and screened for HBV-DNA by PCR. Those with HBV-DNA were diagnosed as OBI and PCR-RFLP technique was performed to examine polymorphisms within their Fas gene.
Results: 352 (9.5%) of 3700 blood samples were HBsAg-/anti-HBc+. HBV-DNA was detected in 57 (16.1%) of 352 HBsAg-/anti-HBc+ samples. Therefore, 57 HBsAg-/anti-HBc+/HBV-DNA+ patients were diagnosed as OBI. Patient and control groups had no significant differences in terms of the studied polymorphisms.
Conclusions: The functional polymorphisms in the promoter region of Fas gene are not associated with OBI. Therefore, it may be concluded that polymorphisms at the -670 position of the Fas gene do not have any critical effects on the immune response against HBV in OBI.

  • Implication for Health policy/practice/research/medical education:
    The role of gene polymorphisms in patients with Hepatitis B virus infection is discussed in this study. Reading this article is suggested to all virolo¬gists, genetic specialists in the field of hepatology.
  • Please cite this paper as:
    Arababadi MK, Mohammadzadeh A, Pourfathollah AA, Kennedy D. Polymorphisms within Fas gene are not associated with occult HBV infection. Hepat Mon. 2011;11(1):23-26.

2011 Kowsar M.P.Co. All rights reserved.

Keywords

Hepatitis B infection Fas Polymorphism HBsAg HBV DNA

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