Protective effect of intermittent clamping of the portal triad in the rat liver on liver ischemia-reperfusion injury
Hepatitis Monthly: ,
11 (6); 445-451 Article Type: Research Article
December 29, 2010
February 5, 2011
M, et al. Protective effect of intermittent clamping of the portal triad in the rat liver on liver ischemia-reperfusion injury,
Online ahead of Print
Background: Intermittent clamping (IC) of the portal triad is an effective method of protecting the liver from ischemia-reperfusion injury (IR). In clinical practice, this method is employed during a resection, but its mechanism is still not clear. Objectives: To evaluate the effect of IC on rat liver and determine its mechanisms. Materials and Methods: Wistar rats were submitted to 60-min IC (cycles of 12-min clamping followed by 4-min reperfusion), and the samples were collected after 1, 6, and 72 hrs of reperfusion. We determined the serum activity of alanine aminotransferase (ALT), and measured the concentration of TNF-α, malondialdehyde (MDA) and myeloperoxidase (MPO) in liver homogenates. The apoptosis of hepatocytes was evaluated immunohistochemically. Results: When compared to the IR rats, the activity of ALT decreased in the IC group in all periods of observation (the highest decrease of ~48% after 1 hr of reperfusion). When compared to the IR group, a statistically significant decrease (p < 0.05) in the TNF-α concentration (~33%) in the IC rats occurred only after 1 hr of reperfusion, and it was accompanied by a decrease in the MPO concentration after 1 and 6 hrs of reperfusion. IC reduces the effects of reactive oxygen species (ROS) activity, which has been confirmed by a statistically significant decrease in MDA concentration by 25%-35% in all studied periods. The limitation of hepatocytes apoptosis due to IC occurs in the early (~26%; p < 0.05) and late (~45%; p < 0.01) phases of reperfusion. Conclusions: The use of IC in early phase of reperfusion brings about a decrease in TNF-α release, which can be related to liver injury due to neutrophil infiltration and apoptotic cell reduction. It seems that the reduction of lipid peroxidation may also limit the liver injury.
Implication for health policy/practice/research/medical education:Animal studies can open new windows to better understanding of pathophysiology of liver ischmia. We suggest reading this article for all pathologists.
Please cite this paper as:Helewski K, Kowalczyk-Ziomek G, Czecior E, Wyrobiec G, Harabin-Slowinska M, Juszko-Piekut M, et al. Protective effect of intermittent clamping of the portal triad in the rat liver on liver ischemia-reperfusion injury. Hepat Mon. 2011;11(6):445-51.
2011 Kowsar M.P.Co. All rights reserved.
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