The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue

AUTHORS

Xue-bing Yan 1 , * , Zhi Chen 2 , Christian Brechot 3

1 Department of Infectious Diseases, the First Affiliated Hospital of Xuzhou Medical College, yxbxuzhou@126.com, China

2 Institute of Infectious Diseases, the First Affiliated Hospital, College of Medical Science, Key Laboratory of Ministry Health, Zhejiang University, China

3 Inserm, France, University Paris sud, Centre Hepato-Biliaire, Hopital Paul Brousse, France

How to Cite: Yan X, Chen Z, Brechot C. The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue, Hepat Mon. Online ahead of Print ; 11(4):255-262.

ARTICLE INFORMATION

Hepatitis Monthly: 11 (4); 255-262
Article Type: Research Article
Received: August 7, 2010
Accepted: December 4, 2010

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: Core protein of the hepatitis C virus (HCV) has an important role in HCV self-replication, pathogenesis and carcinogenesis.
Objectives: To identify the effect of core proteins from different quasispecies of HCV genotype 1b expressed in a HepG2 cell line on human gene expression profiles.
Materials and Methods: Core protein eukrocytic expression plasmids (pEGFP-N1) containing different quasispecie core protein genes of genotypes 1b HCV derived from of HCV-related hepatocellular carcinoma (HCC) tumoral tissue (T) and non-tumoral tissue (NT) were constructed and then transfected to HepG2 cell line. The gene expression spectrum in the cell expression core proteins from T and NT were compared with those in the control by Affymetrix human genome HG-U 133 plus 2.0 microarray.
Results: Different gene expression profiles were acquired between HepG2 expressing core proteins derived from T and NT tissues. Both core proteins caused the modulation of several genes that are up/down-regulated as compared to control, including genes involved in oncogenesis, signal transduction, cell apoptosis, and cell growth cycle regulation. Surprisingly, only one gene-CSNK1A1-was up-regulated by both T and NT core variants.
Conclusions: Core proteins isolated from tumoral or non-tumoral nodules mediate expression of different cellular genes suggesting that variants isolated from different quasispecies may have different biological effects.
Keywords: Hepatitis C virus; Viral core proteins; Genotypes; Microarray analysis; Gene expression


  • Implication for health policy/practice/research/medical education:
    The study of the role of core protein of HCV is recommended for all basic scientists especially those involved in treatment of HCC.
  • Please cite this paper as:
    Yan XB, Chen Z, Brechot C. The differences in gene expression profile induced by genotype 1b hepatitis C virus core isolated from liver tumor and adjacent non-tumoral tissue. Hepat Mon. 2011;11(4):255-262.

© 2011 Kowsar M.P.Co. All rights reserved.


Keywords

Hepatitis C virus Viral core proteins Genotypes Microarray analysis Gene expression

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: