HEV-Capsid Protein Interacts With Cytochrome P4502C8 and Retinol-Binding Protein 4

AUTHORS

Quan Shen? 1 , Wen Zhang? 1 , Yanjun Kang 2 , Yan Chen 3 , Li Cui 4 , * , Zhibiao Yang 3 , Xiuguo Hua 3

1 1) School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai, China.2) School of Medical Technology, Jiangsu University, Jiangsu, China

2 Department of Zoonosis, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, China

3 School of Agriculture and Biology, Shanghai JiaoTong University, China

4 School of Agriculture and Biology, Shanghai JiaoTong University, lcui@sjtu.edu.cn, China

How to Cite: Shen? Q, Zhang? W, Kang Y, Chen Y, Cui L, et al. HEV-Capsid Protein Interacts With Cytochrome P4502C8 and Retinol-Binding Protein 4, Hepat Mon. Online ahead of Print ; 11(11):913-917. doi: 10.5812/kowsar.1735143X.768.

ARTICLE INFORMATION

Hepatitis Monthly: 11 (11); 913-917
Article Type: Research Article
Received: March 1, 2011
Accepted: August 18, 2011
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Abstract

Δ These authors contributed equally

Background: Hepatitis E virus (HEV) is a major causative agent of acute clinical hepatitis in adults throughout much of Asia, the Middle East, and Africa. The lack of an efficient cell culture system for HEV has greatly limited our understanding of the mechanisms of infection, replication, and pathogenicity of this virus. The yeast two-hybridization system is considered to be an efficient method for determining protein-protein interactions and screening interactive proteins associated with host cells.
Objectives: In order to identify the host-cell proteins interacting with the HEV-capsid proteins, a fragment of the HEV-capsid protein p239 (amino acids 368-606) was used as bait; human liver cDNA library was used as a source of host-cell proteins, and the screening was performed using the CytoTrap yeast two-hybrid system.
Materials and Methods: The CytoTrap yeast two-hybrid system, which is also called Sos Recruitment System (SRS), was used to analyze the interaction of the p239 fragment with host-cell proteins.
Results: We isolated 2 proteins, cytochrome P4502C8 (CYP4502C8) and retinol-binding protein 4 (RBP4) after 2 rounds of screening. Co-immunoprecipitation assays showed that both the proteins could bind in vitro to the HEV virion in HepG2 cells.
Conclusions: CYP4502C8 and RBP4 screened from liver cDNA library using the CytoTrap yeast two-hybrid system interact with HEV capsid in vitro.

 


 

Implication for health policy/practice/research/medical education:
The study would provide information to understand the mechanisms of infection and replication.

Please cite this paper as:
Shen Q, Zhang W, Kang Y, Chen Y, Cui L, Yang Z, et al. HEV-Capsid Protein Interacts With Cytochrome P4502C8 and Retinol-Binding
Protein 4. Hepat Mon. 2011; 11(11):913-7. DOI: 10.5812/kowsar.1735143X.768

Keywords

Hepatitis E Virus Protein Interaction Mapping Two-Hybrid System Techniques

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