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The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice

AUTHORS

Elham Torbati 1 , Mojgan Bandehpour 2 , * , Parviz Pakzad 1 , Nariman Mosaffa 1 , Ameneh Koochaki 1 , Bahram Kazemi 1

AUTHORS INFORMATION

1 Microbiology Department, Islamic Azad University, north Tehran Branch, IR Iran

2 1) Cellular and Molecular Research Center. 2) Biotechnology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, m.Bandehpour@sbmu.ac.ir, IR Iran

How to Cite: Torbati E, Bandehpour M, Pakzad P, Mosaffa N, Koochaki A, et al. The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice, Hepat Mon. Online ahead of Print ; 12(6):391-397. doi: 10.5812/hepatmon.6141.

ARTICLE INFORMATION

Hepatitis Monthly: 12 (6); 391-397
Article Type: Research Article
Received: February 16, 2012
Accepted: April 20, 2012
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Abstract

Background: Hepatitis infection represents one of the important causes of morbidity and mortality in developing countries, however there is not any effective vaccine against hepatitis C which is one of the significant problems in vaccine project.
Objectives: The aim of the present study is to evaluate the role of HCV core protein in inducing IFN-Gamma secretion and TCL activities as a vaccine in Balb/C mice.
Material and Methods: Our previous cloned plasmid (HCV Core gene into pETDuet-1) applied for protein expression in bacteria. The expressed and purified recombinant protein together with Freund’s adjuvant was injected to 15 Balb/c mice. The total IgG and IgG2a of immunized mice sera were evaluated after a week. Two weeks after booster injection, we studied the proliferation and IFNγ secretion of spleens, inguinal and popliteal lymph nodes lymphocytes by ELISA and ELISPOT.
Results: The FSFC (Frequency of spot forming cells) of secreting cells of immunized mice with HCV/Core protein and sera IgG2a were considerably higher than the control groups.
Conclusions: The core protein together with proper adjuvant can be a candidate vaccine against of HCV infection.


Implication for health policy/practice/research/medical education:
This study has implication for further researches and vaccine development and it is also useful for medical researchers and education specially immunologists.
Please cite this paper as:
Torbati E, Bandehpour M, Pakzad P, Mosaffa N, Kazemi B. The Evaluation of Hepatitis C Virus Core Antigen in Immunized Balb/C Mice. Hepat Mon. 2012;12(6): 391-397. DOI: 10.5812/hepatmon.6141

Copyright © 2012 Kowsar Corp. All rights reserved.


Keywords

Hepacivirus Core protein Recombinant Proteins

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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