Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population
Hepatitis Monthly: November 6, 2012, 12 (11); 7283
November 6, 2012
Article Type: Research Article
July 17, 2012
September 15, 2012
M , Mohebbi
S R, Naghoosi
H , Tahaei
M E, et al. Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population,
chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-? and its receptor (IFN-? R) are critical in the pathogenesis of infection.
The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-? R1) and chronic HBV infection.
Materials and Methods:
Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.
The results indicated that both SNPs frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.
Presence of G allele at position -611 within promoter of IFN-? R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.
Hepatitis B Virus; Single Nucleotide Polymorphism; Interferon Gamma Receptor; Polymorphism, Restriction Fragment Length
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