Immunogenicity of Multi-Epitope DNA and Peptide Vaccine Candidates Based on Core, E2, NS3 and NS5B HCV Epitopes in BALB/c Mice

Leila Pishraft Sabet; Tahereh Taheri; Arash Memarnejadian; Talat Mokhtari Azad; Fatemeh Asgari; Ramin Rahimnia; Seyed Moayed Alavian; Sima Rafati; Katayoun Samimi Rad

doi:10.5812/hepatmon.22215

Immunogenicity of Multi-Epitope DNA and Peptide Vaccine Candidates Based on Core, E2, NS3 and NS5B HCV Epitopes in BALB/c Mice

Leila Pishraft Sabet ² , Tahereh Taheri ³ , Arash Memarnejadian ⁴ , Talat Mokhtari Azad ¹ , Fatemeh Asgari ¹ , Ramin Rahimnia ⁵ , Seyed Moayed Alavian ⁷ , Sima Rafati ³ and Katayoun Samimi Rad ^{1
, *}

Authors Information

² Razi Vaccine and Serum Research Institute, Karaj, IR Iran

³ Molecular Immunology and Vaccine Research Laboratory, Pasteur Institute of Iran, Tehran, IR Iran

⁴ Hepatitis and HIV Laboratory, Pasteur Institute of Iran, Tehran, IR Iran

¹ Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran

⁵ Department of Nano Medicine, School of Advanced Technologies in Medicine, Tehran, IR Iran

⁷ Middle East Liver Disease Center (MELD), Tehran, IR Iran

Article information

Hepatitis Monthly: October 29, 2014, 14 (10); 22215
Published Online: October 14, 2014
Article Type: Research Article
Received: July 20, 2014
Accepted: September 20, 2014
DOI: 10.5812/hepatmon.22215

To Cite: Pishraft Sabet L, Taheri T, Memarnejadian A, Mokhtari Azad T, Asgari F, et al. Immunogenicity of Multi-Epitope DNA and Peptide Vaccine Candidates Based on Core, E2, NS3 and NS5B HCV Epitopes in BALB/c Mice, Hepat Mon. 2014 ; 14(10):22215. doi: 10.5812/hepatmon.22215.

Abstract

Materials and Methods: In this experimental study, multi-epitope DNA- and peptide-based vaccines for HCV infection harboring immunodominant CD8+ T cell epitopes (HLA-A2 and H2-Dd) from Core (132-142), NS3 (1073-1081) and NS5B (2727-2735), a Th CD4+ epitope from NS3 (1248-1262) and a B-cell epitope from E2 (412-426) were designed. Multi-epitope DNA and peptide vaccines were tested in two regimens as heterologous DNA/peptide (group 1) and homologous peptide/peptide (group 2) prime/boost vaccine in BALB/c mice model. Electroporation was used for delivery of the DNA vaccine. Peptide vaccine was formulated with Montanide ISA 720 (M720) as adjuvant. Cytokine assay and antibody detection were performed to analyze the immune responses.

Results: Mice immunized with multi-epitope peptide formulated with M720 developed higher HCV-specific levels of total IgG, IgG1 and IgG2a than those immunized with multi-epitope DNA vaccine. IFN-? levels in group 2 were significantly higher than group 1 (i.e. 3 weeks after the last immunization; 37.61 2.39 vs. 14.43 0.43, P < 0.05). Moreover, group 2 had a higher IFN-?/IL-4 ratio compared to group 1, suggesting a shift toward Th1 response. In addition, in the present study, induced immune responses were long lasting and stable after 9 weeks of the last immunization.

© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Leila Pishraft Sabet: [PubMed] [Scholar]
Tahereh Taheri: [PubMed] [Scholar]
Arash Memarnejadian: [PubMed] [Scholar]
Talat Mokhtari Azad: [PubMed] [Scholar]
Fatemeh Asgari: [PubMed] [Scholar]
Ramin Rahimnia: [PubMed] [Scholar]
Seyed Moayed Alavian: [PubMed] [Scholar]
Sima Rafati: [PubMed] [Scholar]
Katayoun Samimi Rad: [PubMed] [Scholar]

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