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Molecular Diagnosis of Entecavir Resistance

AUTHORS

Murat Sayan 1 , *

1 Clinical Laboratory, Faculty of Medicine, Umuttepe Kampus, University of Kocaeli, sayanmurat@hotmail.com, Kocaeli, Turkey

How to Cite: Sayan M. Molecular Diagnosis of Entecavir Resistance, Hepat Mon. Online ahead of Print ; 10(1):42-47.

ARTICLE INFORMATION

Hepatitis Monthly: 10 (1); 42-47
Article Type: Review Article
Received: November 21, 2009
Accepted: January 14, 2010

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Abstract

Entecavir (ETV) is a potent nucleoside analogue against hepatitis B virus (HBV). Because of development of ETV resistance requires at least three amino acid substitutions in HBV polymerase (pol) gene, emergence of ETV resistance is rare (~1%) in nucleoside-naive patients after up to 5 years of treatment. However, it has been suggested that lamivudine (LAM) therapy can preselect for HBV variants associated with resistance to ETV treatment. ETV resistance increased to 51% of patients after 5 years of ETV treatment in LAM refractory patients. The diagnosis of ETV resistance in chronic hepatitis B patients, mainly based on four types of molecular assays: direct sequencing, line probe assay, clonal analysis, and restriction fragment length polymorphism (RFLP) analysis. The applications of other assays are currently more specialized, and their use is more limited. The utility of these assays and their performance characteristics are reviewed below. Briefly, the monitoring of drug-resistant variants is important in the elucidation of the prevalence and mechanisms of resistance development and for the more effective management of treatment options.

Keywords

Entecavir Chronic Hepatitis B Nucleoside Analogue Entecavir Resistance Molecular Diagnosis

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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