E-Selectin Gene Polymorphisms in Iranian Chronic Hepatitis B Patients

AUTHORS

Mehrdad Hajilooi 1 , Amir-Houshang Mohammad Alizadeh 2 , Mitra Ranjbar 2 , Farahnaz Fallahian 2 , Seyed Moayed Alavian 3 , *

1 Department of Immunology, Hamedan University of Medical Sciences, Hamedan, IR.Iran

2 Department of Gastroenterology, Shaheed Beheshti University of Medical Sciences, Tehran, IR.Iran

3 Professor of Gastroenterology and Hepatology, Baqiyatallah Research Center for Gastroenterology and Liver Disease (BRCGL), Baqiyatallah University of Medical Sciences, [email protected], Tehran, IR.Iran

How to Cite: Hajilooi M, Alizadeh A, Ranjbar M, Fallahian F, Alavian S. E-Selectin Gene Polymorphisms in Iranian Chronic Hepatitis B Patients, Hepat Mon. Online ahead of Print ; 7(4):211-216.

ARTICLE INFORMATION

Hepatitis Monthly: 7 (4); 211-216
Article Type: Research Article
Received: November 17, 2007
Accepted: January 5, 2008

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background and Aims: The aim of this study is to detect the substitutions Ser128Arg (A128C) and Leu554Phe (T554C) which responsible for E-selectin polymorphisms in patients with chronic hepatitis B and healthy controls. We investigated association of the Ser128Arg, Leu554Phe gene polymorphisms in the E-selectin gene as prototypical inflammatory molecules for susceptibility to chronic hepatitis B.

Methods: Sixty-three patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to clinic. Genomic DNA was isolated from anti-coagulated peripheral blood Buffy coat using Miller's salting-out method. The presence of the E-selectin gene polymorphisms was determined by using polymerase chain reaction amplification refractory mutation system (ARMS).

Results: Distribution of E-selectin 128 (A+C-, A+C+, A-C+) genotypes and E-selectin genotype 554 (C+T-, T+C-, C+T+) genotypes were not statistically significant different in chronic hepatitis B patients and controls by Chi-square test (P=0.41 & 0.96, respectively). Also, Two groups had not statistically significant difference in distribution of frequencies of allele 128 A by Chi-square test (P=0.41), 128 C (P=0.15), allele 554 C (P=0.85), and allele 554 T (P=0.76). Carrying allele 128 A (OR=0.587, 95% CI=0.162-2.124), 128 C (OR=1.526, 95% CI=0.849-2.745), 554 C (OR=1.245, 95% CI=0.128-12.089), and allele 554 T (OR=0.880, 95% CI=0.384-2.014) were not risk factors for susceptibility to chronic hepatitis B infection.

Conclusions: Carrying E-selectin gene polymorphisms of Ser128Arg and Leu554Phe are not risk factors for susceptibility to chronic hepatitis B.

Keywords

E-Selectin Gene Chronic Hepatitis B Gene Polymorphisms

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: