Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population

AUTHORS

Sayyad Khanizadeh 1 , Mehrdad Ravanshad 1 , * , Seyed Reza Reza Mohebbi 2 , * , Hamed Naghoosi 2 , Mohamad Ebrahim Abrahim Tahaei 2 , Seyed Dawood Dawood Mousavi Nasab 1 , Sara Romani 2 , Pedram Azimzadeh 2 , Azar Sanati 2 , Mohammad Reza Reza Zali 2

1 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, [email protected], IR Iran

2 Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, [email protected], IR Iran

Corresponding Authors:

How to Cite: Khanizadeh S, Ravanshad M , Mohebbi S R, Naghoosi H , Tahaei M E, et al. Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population, Hepat Mon. 2012 ; 12(11):7283. doi: 10.5812/hepatmon.7283.

ARTICLE INFORMATION

Hepatitis Monthly: 12 (11); 7283
Published Online: November 6, 2012
Article Type: Research Article
Received: July 17, 2012
Accepted: September 15, 2012
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Abstract

Background: chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-? and its receptor (IFN-? R) are critical in the pathogenesis of infection.

Objectives: The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-? R1) and chronic HBV infection.

Materials and Methods: Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.

Results: The results indicated that both SNPs frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.

Conclusions: Presence of G allele at position -611 within promoter of IFN-? R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.

Keywords

Hepatitis B Virus Single Nucleotide Polymorphism Interferon Gamma Receptor Polymorphism, Restriction Fragment Length

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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