Distribution of IL28B Genotypes in Iranian Patients with Chronic Hepatitis C and Healthy Individuals

AUTHORS

Heidar Sharafi 2 , Ali Pouryasin 3 , * , Seyed Moayed Alavian 4 , Bita Behnava 4 , Maryam Keshvari 5 , Shima Salimi 4 , Leila Mehrnoush 4 , Ahmad Fatemi 6

2 Tehran Hepatitis Cohort (THC) Study Center, Iran

3 Department of Genetics, Islamic Azad University of Arsanjan, [email protected], Iran

4 Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences, Iran

5 Iranian Blood Transfusion Organization, Iran

6 Department of Hematology, Tehran University of Medical Sciences, Iran

How to Cite: Sharafi H , Pouryasin A , Alavian S M , Behnava B , Keshvari M, et al. Distribution of IL28B Genotypes in Iranian Patients with Chronic Hepatitis C and Healthy Individuals, Hepat Mon. 2012 ; 12(12):8387. doi: 10.5812/hepatmon.8387.

ARTICLE INFORMATION

Hepatitis Monthly: 12 (12); 8387
Published Online: December 21, 2012
Article Type: Research Article
Received: July 24, 2012
Accepted: October 1, 2012
Crossmark

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: IL28B polymorphism is recognized as one of the most prominent predictors of hepatitis C spontaneous and treatment-induced clearance. Interestingly, the favorable genotypes of IL28B are found to be more frequent in Asian ethnicity than Caucasian and African populations, respectively. A few studies reported that there is a mysterious association between the IL28B polymorphism and the hepatitis C virus (HCV) genotype in patients with chronic hepatitis C but they did not give any reason for this phenomenon.

Objectives: The foremost purpose of this study was to compare the distribution of IL28B genotypes between Iranian healthy individuals and patients with chronic hepatitis C.

Patients and Methods: In this study, 921 patients with chronic hepatitis C and 142 healthy individuals were included. The IL28B rs12979860 and rs8099917 polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results: The frequency of IL28B rs12979860 CC, CT, and TT genotypes in chronic hepatitis C patients was 38%, 48.8%, and 13.2% and in healthy individuals was 43.7%, 48.6%, and 7.7%. Also, the frequency of IL28B rs8099917 TT, GT, and GG genotypes in chronic hepatitis C patients was 58.3%, 37.1%, and 4.6% and in healthy individuals was 64.1%, 32.4% and 3.5%. The differences in the distribution of IL28B rs12979860 and rs8099917 genotypes between patients with chronic hepatitis C and healthy individuals were not statistically significant. When we compared the distribution of IL28B genotypes between the healthy group and the HCV infected patients by HCV genotype, we found 9.8% higher frequency of rs12979860 CC genotype in the healthy individuals than HCV genotype 1 infected patients (P = 0.03) however there was no significant difference in the distribution of rs12979860 genotypes between the healthy and HCV genotype 3 infected groups (P = 0.46).

Conclusions: It seems that the impact of IL28B polymorphism on the spontaneous clearance of HCV genotype 1 is more prominent than HCV genotype 3 which results in the observation of higher rs12979860 C allele frequency in chronic hepatitis C patients with HCV genotype 3 than HCV genotype 1.

Keywords

Polymorphism, Genetic Hepatitis C IL28B Genotype, Human Persian Gulf Iran

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: